![adobe photoshop cs6 extended serial number 2015 adobe photoshop cs6 extended serial number 2015](http://www.newdesignfile.com/postpic/2013/02/adobe-audition-cc-mac_335983.jpg)
![adobe photoshop cs6 extended serial number 2015 adobe photoshop cs6 extended serial number 2015](http://3.bp.blogspot.com/-oLnroGTBv_w/URHdToUGQhI/AAAAAAAAADg/kKWivwIwRYU/s1600/Adobe+Photoshop+CS6+13.0.1+Extended+Pull+01.jpg)
( A) Chromatogram of the analytical C18 RP-HPLC fractionation of the Egyptian snake venom into 24 fractions. aegyptia venom primary fractions on OF1 mice sperm motility. mass spectrometry peptide sequencing proteomics sperm motility.Įffect of W. Snake venom Walterinnesia aegyptia bioactive components drug screening. Its fluorescently-labelled analog predominantly marks the flagellum, a localization in agreement with a receptor that controls motility function. The synthetic peptide shows promising activation of sperm motility from a variety of species, including humans. Moreover, it has strong homology with several other hitherto cloned Elapidae and Viperidae snake toxins suggesting that it belongs to a family of compounds able to regulate sperm function. The peptide is 57 amino acid residues long and contains three disulfide bridges and was found to be identical to the previously cloned Wa Kunitz-type protease inhibitor II (Wa Kln-II) sequence. Walterospermin was de novo sequenced using a combination of matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF MS/MS) and liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF MS/MS) following reduction, alkylation, and enzymatic proteolytic digestion with trypsin, chymotrypsin or V8 protease. Using RP-HPLC and cation exchange chromatography, we identified a new toxin of 6389.89 Da (termed walterospermin) that activates sperm motility. Herein, we screened for bioactive compounds from the venom of the Egyptian black snake Walterinnesia aegyptia (Wa) that possess the property to activate sperm motility in vitro from male mice OF1. Since sperm cells express a wide variety of ion channels and membrane receptors, required for the control of cell motility and acrosome reaction, two functions that are defective in infertility issues, animal venoms should contain interesting compounds capable of modulating these two essential physiological functions. They are known to target at least two important pharmacological classes of cell surface receptors: ion channels and G protein coupled receptors. 11 Archamps BioPark, 260 Avenue Marie Curie, Archamps Technopole, F-74166 Saint Julien en Genevois, France.Īnimal venoms are small natural mixtures highly enriched in bioactive components.10 Université de Nice Sophia-Antipolis, LabEx «Ion Channels, Science & Therapeutics», F-06560 Valbonne, France.9 L'institut du thorax, INSERM, CNRS, UNIV NANTES, F-44007 Nantes, France.8 Université Grenoble Alpes, 38000 Grenoble, France.7 Institut Pour l'Avancée des Biosciences (IAB), INSERM U1209, CNRS UMR 5309, 38700 La Tronche, France.6 PROMETHEE Proteomic Platform, Institut de Biologie et de Pathologie, IAB, CHU Grenoble Alpes, 38000 Grenoble, France.5 PROMETHEE Proteomic Platform, INSERM U1055, 38041 Grenoble, France.4 PROMETHEE Proteomic Platform, University Grenoble Alpes, LBFA et BEeSy, 38041 Grenoble, France.3 Smartox Biotechnology, 6 rue des Platanes, 38120 Saint-Egrève, France.2 Zoology Department, Faculty of Science, Minia University, El-Minia 61519, Egypt.1 Department of Cellular and Integrative Physiology, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USA.